Figure 9.
![Figure 9. Cdk5 phosphorylates Drp1 at SerCDK to disrupt MT targeting of Drp1-x01. (A) GFP-Drp1-101/111 wild type (WT) and SerCDKAla (SA) mutant was coexpressed ± Cdk5/p35 in HeLa cells and total lysates were analyzed for Drp1 SerCDK phosphorylation with a phospho-preferring antibody. The ratio of phosphorylated (pDrp1) to total Drp1 is listed in arbitrary units. (B and C) HeLa cells expressing GFP-Drp1 ± Cdk5/p35 were treated with taxol (10 µM, 6 h) and analyzed for colocalization of GFP and β-tubulin immunofluorescence signals. Representative epifluorescence images are shown in B, whereas colocalization scores are plotted in C (means ± SEM [up]/SD [down] of 53–108 cells). *, P = 10−22 comparing Drp1-101 wild type ± Cdk5/p35.](https://cdn.rupress.org/rup/content_public/journal/jcb/201/7/10.1083_jcb.201210045/5/jcb_201210045_fig9.jpeg?Expires=1767758769&Signature=a1V-coYBgeUGOHEYtPRxa9EKRTxIGrnynpRa6mgk0y0toPlsGwvQBQO0s2R17Xm8wZRVf8gm8SOHjKxCMW3TxPNsr7SS5FpqZlT5R6j-1j2~hmqu5lZtGmm7KxV39tYSvt4Y5ObXEIezyZ44Hryl9hJnKG~eN~IiggDnbYnG4OpAqDgO~-zOIGYbqYYq7OwjrvIQHwsctilO8oP2NQ367-zvVsWtguRlbTM7QMfPkRchLsa2WyaJG2hxQ92kdHa2njYfzw0ndCfGmiwlvdNXwZ7OOJdIgoaJmna~7W5FHW~dcg6lz6rQutJpPbfrd6qrARlDnCc11eYBdlWgTIbzWg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cdk5 phosphorylates Drp1 at SerCDK to disrupt MT targeting of Drp1-x01. (A) GFP-Drp1-101/111 wild type (WT) and SerCDKAla (SA) mutant was coexpressed ± Cdk5/p35 in HeLa cells and total lysates were analyzed for Drp1 SerCDK phosphorylation with a phospho-preferring antibody. The ratio of phosphorylated (pDrp1) to total Drp1 is listed in arbitrary units. (B and C) HeLa cells expressing GFP-Drp1 ± Cdk5/p35 were treated with taxol (10 µM, 6 h) and analyzed for colocalization of GFP and β-tubulin immunofluorescence signals. Representative epifluorescence images are shown in B, whereas colocalization scores are plotted in C (means ± SEM [up]/SD [down] of 53–108 cells). *, P = 10−22 comparing Drp1-101 wild type ± Cdk5/p35.
