Figure 4.
![Figure 4. Fluorescence recovery of Drp1 is modulated by MT association and GTP hydrolysis. (A–F) A square in the cytosol of HeLa cells expressing the indicated GFP-tagged proteins was bleached and fluorescence recovery was monitored every 5 s for up to 8 min. Frames of representative time series are shown in A and D, whereas recovery curves and kinetic parameters derived from biexponential fits of the recovery curves (50% recovery time, t1/2; mobile fraction) are plotted in B, C, E , and F (means ± SEM [up]/SD [down] of 6–8 cells). ○, P < 0.05; Ø, P < 0.01; ⊗, P < 0.005; *, P < 0.0001.](https://cdn.rupress.org/rup/content_public/journal/jcb/201/7/10.1083_jcb.201210045/5/jcb_201210045_fig4.jpeg?Expires=1767711012&Signature=3vLH1Oyj6bMhkCN~3~FaRx3pGlWh4Og3u5i~6mSUad3Z4OTPDoLQHfY8N3VKB7jFwTxPYb3P8PSLKQK5ifWH0psCR7Wo6eFvetmXh00f~caNw6j1y0IjB8LX676jMwFQvEqoy16H~5K8BWU0wxWlXNyF-ImeC~l9x~GPFr~Ii8tPddilBLMXLUxUmVp-2uIlwcjodLL804dCyijVvheVuJe7jz0cePurL40oFMAUe1sYRRuExP82g7fLJzwfpWh2I04k4HrAPv7gANN3TqlvOK5~L5pvdhmpI6K-g5AfR1NY0CufIe49n-LYDTzYB1QPb342WixHnOJFd-Qqd6~0bw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fluorescence recovery of Drp1 is modulated by MT association and GTP hydrolysis. (A–F) A square in the cytosol of HeLa cells expressing the indicated GFP-tagged proteins was bleached and fluorescence recovery was monitored every 5 s for up to 8 min. Frames of representative time series are shown in A and D, whereas recovery curves and kinetic parameters derived from biexponential fits of the recovery curves (50% recovery time, t1/2; mobile fraction) are plotted in B, C, E , and F (means ± SEM [up]/SD [down] of 6–8 cells). ○, P < 0.05; Ø, P < 0.01; ⊗, P < 0.005; *, P < 0.0001.
