Figure 7. p37 and p47 control spindle orientation in human cells. (A) Time-lapse recording of HeLa Kyoto cells expressing mCherry::H2B and GFP::α-tubulin treated with either control siRNA or siRNA targeting p37 and p47. (B) Spindle rotation during mitosis, expressed as percentage of cells in control siRNA or p37/p47 siRNA–treated cells. The two distributions are significantly different (χ2 test). (C) Mean spindle angle (α°) ± SEM in control siRNA, p37 siRNA, p47 siRNA, or p37 and p47 siRNA–treated HeLa cells. Measurements were done on fixed cells immunostained with α- and γ-tubulin. (D) Quantification of spindle rotation (z-axis) during mitosis expressed as percentage of cells, in control siRNA or p37 siRNA–treated cells induced or not for the expression of an siRNA-resistant form of p37 (p37RNAiR). **, P < 0.01 (χ2 test) for the indicated differences; #, P < 0.01 compared with siCtrl. Numbers of cells analyzed indicated in italic (siCtrl-sip37/p47).
Figure 7.

p37 and p47 control spindle orientation in human cells. (A) Time-lapse recording of HeLa Kyoto cells expressing mCherry::H2B and GFP::α-tubulin treated with either control siRNA or siRNA targeting p37 and p47. (B) Spindle rotation during mitosis, expressed as percentage of cells in control siRNA or p37/p47 siRNA–treated cells. The two distributions are significantly different (χ2 test). (C) Mean spindle angle (α°) ± SEM in control siRNA, p37 siRNA, p47 siRNA, or p37 and p47 siRNA–treated HeLa cells. Measurements were done on fixed cells immunostained with α- and γ-tubulin. (D) Quantification of spindle rotation (z-axis) during mitosis expressed as percentage of cells, in control siRNA or p37 siRNA–treated cells induced or not for the expression of an siRNA-resistant form of p37 (p37RNAiR). **, P < 0.01 (χ2 test) for the indicated differences; #, P < 0.01 compared with siCtrl. Numbers of cells analyzed indicated in italic (siCtrl-sip37/p47).

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