Regulation of Aurora A centrosomal levels by UBX adaptors is conserved in human cells. (A) Scheme of the main domains of UBXN-2, p37, and p47. UBA, ubiquitin-associating domain; UBX, ubiquitin regulatory X domain; SEP, Shp eyes-closed p47domain. (B) GFP-, p37-GFP–, or p47-GFP–expressing cells stained for γ-tubulin and DNA in prophase or metaphase. Centrosomal localization is observed in 85.7% of the p37-GFP cells (n = 56), 52.9% of the p47-GFP cells (n = 68), and 26.2% of GFP cells (n = 61). The white arrowhead indicates the centrosomes in the GFP panels. (C) Percentage of cells with unseparated centrosomes at NEBD. **, P < 0.01 (χ² test) for the indicated differences; #, P < 0.01 compared with siCtrl. (D) Stills from time-lapse recording of HeLa Kyoto cells expressing mCherry::H2B and GFP::α-tubulin treated with either control siRNA or siRNA targeting p37 and p47. White arrowheads show the separated centrosomes. (E) Control or p37p47 siRNA–treated HeLa cells immunostained with DAPI and Aurora A. (F and G) Mean Aurora A fluorescence intensities at centrosomes in control or p37p47 siRNA–treated HeLa cells, in prophase (F) or metaphase (G).