UBXN-2 regulates centrosomal protein levels and centrosome behavior in a cell cycle–dependent manner. (A, C, and E) Control and ubxn-2(RNAi) embryos stained for α-tubulin, DNA, ZYG-9 (A), TAC-1 (C), or γ-tubulin (E). The insets present a magnified view (6.5 × 6.5 µm) of the centrosomes indicated by white arrowheads. (B, D, and F) Mean fluorescence intensity at centrosomes ± SEM of ZYG-9 (B), TAC-1 (D), or γ-tubulin (F). (G) Analysis of centrosome volume throughout the cell cycle in control or ubxn-2(RNAi) embryos. The strain used (TH65) expresses YFP::α-tubulin. For each centrosome, the volume was normalized to the maximum; the histogram represents the mean ± SEM. (H) Mean growth speed ± SEM of astral microtubule speed in control or ubxn-2(RNAi). The strain used (TH66) expresses EBP2::GFP. (I) Quantification of the number of astral microtubules longer than 3.75 µm per centrosome in control or ubxn-2(RNAi) embryos in prophase. (J) Quantification of the ubxn-2(RNAi) spindle misorientation phenotype observed by DIC in ubxn-2(ok1942)/+ embryos depleted for UBXN-2, TAC-1, or both. **, P < 0.01 for the difference between ubxn-2(RNAi) and ubxn-2(RNAi)/tac-1(RNAi) embryos (χ² test).