Figure 3. AIR-1 centrosomal levels are higher in prophase ubxn-2(RNAi) embryos. (A) Fold change (ubxn-2(RNAi) over control) of mean fluorescence intensities at centrosomes (±SEM) for the indicated proteins. The ratio = 1 is indicated by the horizontal bar. At least 30 embryos were analyzed for each protein. (B, D, and F) Embryos of the indicated genotype immunostained for α-tubulin and DNA, AIR-1 (B and F), and P-AIR1 (D). The insets present a magnified view (6.5 × 6.5 µm) of the centrosomes indicated by white arrowheads. (C, E, and G) Mean fluorescence intensity ± SEM at centrosomes of AIR-1 (C and G) or P-AIR-1 (E). (H) Western blot showing AIR-1 levels from embryonic extract of control and ubxn-2(RNAi) embryos. AIR-1 levels, normalized with tubulin, were 0.98 ± 0.1-fold (SEM, four independent experiments). *, P < 0.05; **, P < 0.01 for the difference between control and RNAi-treated embryos (t test).
Figure 3.

AIR-1 centrosomal levels are higher in prophase ubxn-2(RNAi) embryos. (A) Fold change (ubxn-2(RNAi) over control) of mean fluorescence intensities at centrosomes (±SEM) for the indicated proteins. The ratio = 1 is indicated by the horizontal bar. At least 30 embryos were analyzed for each protein. (B, D, and F) Embryos of the indicated genotype immunostained for α-tubulin and DNA, AIR-1 (B and F), and P-AIR1 (D). The insets present a magnified view (6.5 × 6.5 µm) of the centrosomes indicated by white arrowheads. (C, E, and G) Mean fluorescence intensity ± SEM at centrosomes of AIR-1 (C and G) or P-AIR-1 (E). (H) Western blot showing AIR-1 levels from embryonic extract of control and ubxn-2(RNAi) embryos. AIR-1 levels, normalized with tubulin, were 0.98 ± 0.1-fold (SEM, four independent experiments). *, P < 0.05; **, P < 0.01 for the difference between control and RNAi-treated embryos (t test).

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