Figure 6. Expression of Hook1-Y2H results in loss of the recycling tubular endosomal compartment and the targeting of CIE cargo to EEA1-containing endosomes. (A) HeLa cells expressing endogenous levels of Hook1 (top), overexpressing Hook1-Y2H-HA (middle), or coexpressing Hook1-Y2H-HA and GFP-Rab22a (bottom) were incubated with anti-CD147 antibody for 1 h at 37°C to allow internalization of bound antibodies. Hook1-Y2H was localized using a rabbit anti-HA antibody. (B) The degree of restoration of the tubular endosomal network in Hook1-Y2H–expressing cells by GFP-Rab22a overexpression was determined by calculating the percentage of cells in each population exhibiting tubular endosomes. The data presented is the mean of three independent experiments ± SD (error bars). More than 100 cells were scored for each sample. Bars, 10 µm.
Figure 6.

Expression of Hook1-Y2H results in loss of the recycling tubular endosomal compartment and the targeting of CIE cargo to EEA1-containing endosomes. (A) HeLa cells expressing endogenous levels of Hook1 (top), overexpressing Hook1-Y2H-HA (middle), or coexpressing Hook1-Y2H-HA and GFP-Rab22a (bottom) were incubated with anti-CD147 antibody for 1 h at 37°C to allow internalization of bound antibodies. Hook1-Y2H was localized using a rabbit anti-HA antibody. (B) The degree of restoration of the tubular endosomal network in Hook1-Y2H–expressing cells by GFP-Rab22a overexpression was determined by calculating the percentage of cells in each population exhibiting tubular endosomes. The data presented is the mean of three independent experiments ± SD (error bars). More than 100 cells were scored for each sample. Bars, 10 µm.

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