Figure 2.
Figure 2. Hypoxia-induced invadopodia formation requires HIF-1α. (A, left) Cells forming invadopodia in normoxia (N) or 1% O2 (16 h) after transfection with control (C) or HIF-1α siRNAs, n = 3. *, P < 0.01; **, P < 0.05. (right) HIF-1α protein levels after siRNA transfection. (B) Invadopodia activity (degradation) of cells after siRNA treatments and 16 h in normoxia or 1% O2, n = 2. *, P < 0.05; **, P < 0.005. (C) Images from the experiment in A and B. Cells show F-actin at invadopodia (top) and were plated on fluorescent gelatin (bottom) to detect degradation. Graphs show means ± SEM. Bars, 18 µm.

Hypoxia-induced invadopodia formation requires HIF-1α. (A, left) Cells forming invadopodia in normoxia (N) or 1% O2 (16 h) after transfection with control (C) or HIF-1α siRNAs, n = 3. *, P < 0.01; **, P < 0.05. (right) HIF-1α protein levels after siRNA transfection. (B) Invadopodia activity (degradation) of cells after siRNA treatments and 16 h in normoxia or 1% O2, n = 2. *, P < 0.05; **, P < 0.005. (C) Images from the experiment in A and B. Cells show F-actin at invadopodia (top) and were plated on fluorescent gelatin (bottom) to detect degradation. Graphs show means ± SEM. Bars, 18 µm.

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