Cdc7 function regulates replication fork progression. (A) WT and cdc7-as3 cells were synchronized with α-factor for 3 h and 35 min, treated with PP1 for 25 min, and released from α-factor with PP1 and with or without 0.033% MMS. (B) DNA content analysis by FACScan. Analysis of PP1 untreated cells is also shown. (C) Heat maps of BrdU incorporation levels at origins are arranged according to each origin’s published replication timing from early to late (left to right). (D) Aliquots were pulsed with BrdU for the indicated intervals and analyzed by BrdU-IP-chip. Results for segments of chromosomes III and VI are plotted, with origin locations indicated above. Data shown are from a single representative experiment out of two replicates, except data in C, and were calculated from both replicates.