MARK4 or ODF2 depletion leads to accumulation of cells in early stages of ciliogenesis. (A) RPE1 cells stably expressing GFP-Rab8a were treated with the indicated siRNAs. Cells, grown in medium with 10% serum (unstarved) or serum starved for 24 h were stained for γ-tubulin, acetylated tubulin (acetyl. tub.), and DNA. Representative fluorescence micrographs are shown for the localization patterns of GFP-Rab8a. The left images show merged images. Centrosomal regions are shown at higher magnification on the right. (B) Quantification of A. One representative experiment out of three is shown.