Figure 5. Endocytic cortical corralling is required for focused exocytic pole formation. Endo- and exocytic vesicles are marked by Abp1-RFP (red) and GFP-Sec4 (cyan), respectively. (A) In silico and in vivo analyses of the distributions of exocytic cluster size for polarized WT cells (red) compared with endocytic mutants such as sla2Δ (purple), sla1Δ bbc1Δ (blue), rvs167Δ rvs161Δ cells (pink), ede1Δ (black), and clc1Δ (orange) cells. The mean pole diameter is significantly larger in mutants (except for in silico rvs167Δ rvs161Δ) compared with WT (P < 0.01). The black bars indicate the mean and SD. (B) Kymograph of polarized WT cells in vivo (top) displays focused exocytic pole (cyan) during polarization corralled by endocytic vesicles (red). Endocytic mutants defective in endocytic corralling such as sla1Δ bbc1Δ (bottom) and rvs167Δ rvs161Δ (middle) display depolarized endocytic vesicles (red) and wider exocytic poles (cyan).
Figure 5.

Endocytic cortical corralling is required for focused exocytic pole formation. Endo- and exocytic vesicles are marked by Abp1-RFP (red) and GFP-Sec4 (cyan), respectively. (A) In silico and in vivo analyses of the distributions of exocytic cluster size for polarized WT cells (red) compared with endocytic mutants such as sla2Δ (purple), sla1Δ bbc1Δ (blue), rvs167Δ rvs161Δ cells (pink), ede1Δ (black), and clc1Δ (orange) cells. The mean pole diameter is significantly larger in mutants (except for in silico rvs167Δ rvs161Δ) compared with WT (P < 0.01). The black bars indicate the mean and SD. (B) Kymograph of polarized WT cells in vivo (top) displays focused exocytic pole (cyan) during polarization corralled by endocytic vesicles (red). Endocytic mutants defective in endocytic corralling such as sla1Δ bbc1Δ (bottom) and rvs167Δ rvs161Δ (middle) display depolarized endocytic vesicles (red) and wider exocytic poles (cyan).

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