![Figure 7. Exocyst function is required for large particle uptake. (A) Expression of Exo70 rescues large particle uptake defect in Cdc42-depleted cells. Control (shScr) and Cdc42 (shCdc42)-depleted COS1 cells were transfected with FLAG-Exo70 or a control plasmid and challenged with 4.5-µm beads. Uptake was quantified microscopically. (B) Exo70 expression does not rescue large particle uptake defect in Rac1-depleted cells. Control and Rac1 (Rac1 shRNA [shRac1])-depleted COS1 cells, expressing FLAG-Exo70, were challenged with 4.5-µm beads, and uptake was quantified. (C) Large particle uptake defect in NWASP−/− cells is not rescued by Exo70 expression. NWASP+/+ and NWASP−/− cells were transfected with FLAG-Exo70 or a control plasmid and challenged with 4.5-µm beads. Uptake was quantified microscopically. (D and E) Exo70 depletion leads to defective large particle uptake. Control (siGLO) and Exo70 (siExo70)-depleted HeLa cells (D) or FcγRIIA-expressing HeLa cells (E) were challenged with invasin-coated (D) or IgG-coated (E) 4.5-µm beads, and uptake was quantified microscopically. (F and G) Exo70 depletion does not interfere with internalization of small particles. Control (siGLO) and Exo70 (siExo70)-depleted HeLa cells (F) or FcγRIIA-expressing HeLa cells (G) were challenged with invasin-coated (D) or IgG-coated (E) 1.5-µm beads, and uptake was quantified microscopically. Error bars represent SEM. ***, P < 0.001.](https://cdn.rupress.org/rup/content_public/journal/jcb/200/1/10.1083_jcb.201204090/4/jcb_201204090_fig7.jpeg?Expires=1767782546&Signature=cbErl77owoJBk9jTrwhdAOENb2Qep05lesme2SUYqDGQSENeLhrFGbUdkmJ1NRiui4jbLaR2lMWpNuVQlB7KkP5xlMCKoO7vCLPKVt1qf-vqCHKkM8txpZO~ItnTmpLQ1UPYHapYbGLL~VLH08WXYS8Oog1hHMzd8lcn4Yaz2c~0l4TaHx~15H9mJWgguuVsN4F4uySb9K4A92bP4b5CLZVnR1ypEZ5Sy056-aE6jmmcVepGsmEF83nDVxbZE0X2wM~OgCV6VdBlI~Rm71fUe2R2nc-2ZxWbDsgm4~b0Gl-h4Kd26ZCImWVovSF~Zhr8s8Z9RR7nQxl2AlBGS~0UgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Exocyst function is required for large particle uptake. (A) Expression of Exo70 rescues large particle uptake defect in Cdc42-depleted cells. Control (shScr) and Cdc42 (shCdc42)-depleted COS1 cells were transfected with FLAG-Exo70 or a control plasmid and challenged with 4.5-µm beads. Uptake was quantified microscopically. (B) Exo70 expression does not rescue large particle uptake defect in Rac1-depleted cells. Control and Rac1 (Rac1 shRNA [shRac1])-depleted COS1 cells, expressing FLAG-Exo70, were challenged with 4.5-µm beads, and uptake was quantified. (C) Large particle uptake defect in NWASP−/− cells is not rescued by Exo70 expression. NWASP+/+ and NWASP−/− cells were transfected with FLAG-Exo70 or a control plasmid and challenged with 4.5-µm beads. Uptake was quantified microscopically. (D and E) Exo70 depletion leads to defective large particle uptake. Control (siGLO) and Exo70 (siExo70)-depleted HeLa cells (D) or FcγRIIA-expressing HeLa cells (E) were challenged with invasin-coated (D) or IgG-coated (E) 4.5-µm beads, and uptake was quantified microscopically. (F and G) Exo70 depletion does not interfere with internalization of small particles. Control (siGLO) and Exo70 (siExo70)-depleted HeLa cells (F) or FcγRIIA-expressing HeLa cells (G) were challenged with invasin-coated (D) or IgG-coated (E) 1.5-µm beads, and uptake was quantified microscopically. Error bars represent SEM. ***, P < 0.001.
