Acetylation and glucose stimulation modulate K8 site-specific phosphorylation. (A) BHK-21 cells were transfected with the indicated plasmids followed by analysis of the total cell lysates by immunoblotting for total and phospho-K8 (pS74 and pS432). (B) Quantification of the immunoblot data shown in A from three separate experiments. **, P < 0.01; one-way analysis of variance. The results are presented as the mean and the standard deviation. (C) HepG2 cells were grown under normal (4.5g/l glucose) culture conditions (Control) or were glucose starved for 5 h, and then restimulated with glucose-containing medium (4.5g/l) for 1 h before fixation and immunostaining for total and K8 pS74. Bars, 20 µm.