Figure 6. Opg-independent inhibition of osteoclastogenesis by Wnt3a. (A) Quantification of TRAP-positive multinucleated cells generated after 7 d of culture in the presence of Wnt3a and/or an anti-Opg antibody. Error bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences compared with controls. (B) Quantification of TRAP-positive multinucleated cells generated from unsorted or CD11b-purified bone marrow cells after 7 d of culture in the presence of Wnt3a. Error bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences compared with controls. (C) qRT-PCR expression analysis of the indicated genes in CD11b-purified bone marrow cells after treatment with 200 ng/ml Wnt3a for 6 h relative to untreated cells. Error bars represent mean ± SD (n = 4). Asterisks indicate statistically significant differences compared with controls.
Figure 6.

Opg-independent inhibition of osteoclastogenesis by Wnt3a. (A) Quantification of TRAP-positive multinucleated cells generated after 7 d of culture in the presence of Wnt3a and/or an anti-Opg antibody. Error bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences compared with controls. (B) Quantification of TRAP-positive multinucleated cells generated from unsorted or CD11b-purified bone marrow cells after 7 d of culture in the presence of Wnt3a. Error bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences compared with controls. (C) qRT-PCR expression analysis of the indicated genes in CD11b-purified bone marrow cells after treatment with 200 ng/ml Wnt3a for 6 h relative to untreated cells. Error bars represent mean ± SD (n = 4). Asterisks indicate statistically significant differences compared with controls.

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