Compensatory induction of Fzd genes in Wnt3a-treated Fzd8-deficient osteoclast cultures. (A) qRT-PCR expression analysis of the indicated genes in wild-type and Fzd8-deficient bone marrow cultures differentiated in the presence of M-Csf and Rankl with or without Wnt3a as indicated. Error bars represent mean ± SD (n = 4). Asterisks indicate statistically significant differences compared with control-treated cells. Number signs indicate statistically significant differences between wild-type and Fzd8-deficient cultures. (B) qRT-PCR expression analysis of the indicated genes in Fzd8-deficient marrow-flushed bones relative to wild-type littermates. Error bars represent mean ± SD (n = 4).