Figure 2. Phenotypic analysis of Fzd8-deficient mice. (A) Von Kossa/van Gieson staining of spine sections from wild-type and Fzd8-deficient mice at the indicated ages. Histomorphometric quantification of the trabecular bone volume (BV/TV) is given on the right. Bars, 1 mm. (B) Quantification of osteoblast number per bone perimeter (Ob.N/B.Pm) and bone formation rate per bone surface (BFR/BS) in 24-wk-old wild-type and Fzd8-deficient mice. (C) Alizarin red staining of primary calvarial osteoblasts from wild-type and Fzd8-deficient mice after 10 d of differentiation (left) and Western blotting with the indicated antibodies after administration of Wnt5a at day 10 of differentiation (right). The black arrows indicate the position and molecular weight of the nearest marker. All error bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences between the two groups.
Figure 2.

Phenotypic analysis of Fzd8-deficient mice. (A) Von Kossa/van Gieson staining of spine sections from wild-type and Fzd8-deficient mice at the indicated ages. Histomorphometric quantification of the trabecular bone volume (BV/TV) is given on the right. Bars, 1 mm. (B) Quantification of osteoblast number per bone perimeter (Ob.N/B.Pm) and bone formation rate per bone surface (BFR/BS) in 24-wk-old wild-type and Fzd8-deficient mice. (C) Alizarin red staining of primary calvarial osteoblasts from wild-type and Fzd8-deficient mice after 10 d of differentiation (left) and Western blotting with the indicated antibodies after administration of Wnt5a at day 10 of differentiation (right). The black arrows indicate the position and molecular weight of the nearest marker. All error bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences between the two groups.

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