Specific and efficient knockdown of Rbfox3 by siRNAs. (A) Location of the siRNAs specifically targeting Rbfox3-full. Two bars under Rbfox3-full indicate the regions targeted by the siRNAs (siRbfox3). (B) Schematic outline of the strategy to isolate transfected cells from embryos. Neural tube electroporation with siRNAs and the GFP expression construct (left), visualization of GFP signal in an embryo (middle), and FACS of the dissociated embryonic cells (right) are shown. Before cells are dissociated from the embryo, its head and tail were trimmed away (white lines in the middle panel). Typically ∼1% of total dissociated cells are GFP⁺. FSC, forward scatter. (C) Specificity of siRbfox3 for Rbfox3-full. Total RNAs isolated from the GFP⁺ cells electroporated with indicated siRNAs were analyzed by RT-PCR for the indicated mRNAs. Actin mRNA serves as a control. Because the data in panel C of this figure and Fig. 7 A were from the same experiment, the “Actin” panels in these two figures are the same.