Figure 1. Three types of actin SFs. U2OS human osteosarcoma cells were plated on 10 µg/ml fibronectin-coated coverslips and allowed to attach and spread for 4 h before fixation (Hotulainen and Lappalainen, 2006). In the immunofluorescence image, antiphosphotyrosine was used as a marker for focal adhesions (red), phalloidin was used for F-actin SFs (green), and the nucleus (blue) was detected by DAPI. This single cell exhibits the three main types of actin SFs: (transverse) arcs, dorsal SFs, and ventral SFs. (inset) Schematic drawing depicting the SF subtypes.
Figure 1.

Three types of actin SFs. U2OS human osteosarcoma cells were plated on 10 µg/ml fibronectin-coated coverslips and allowed to attach and spread for 4 h before fixation (Hotulainen and Lappalainen, 2006). In the immunofluorescence image, antiphosphotyrosine was used as a marker for focal adhesions (red), phalloidin was used for F-actin SFs (green), and the nucleus (blue) was detected by DAPI. This single cell exhibits the three main types of actin SFs: (transverse) arcs, dorsal SFs, and ventral SFs. (inset) Schematic drawing depicting the SF subtypes.

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