CUPS is distinct from PAS. (A) Yeast cells coexpressing Grh1-GFP and mCherry-Atg8 were cultured in growth medium and either nutrient starved for 4 h or treated with 0.4 µg/ml rapamycin in nutrient-rich medium for 3 h. (B) Yeast cells coexpressing Grh1-GFP and Ape1-mCherry were cultured in growth medium or starved for the indicated time points and visualized by fluorescence microscopy. Bar, 2 µm.