Figure 4. Grh1 colocalizes with Vps23 (ESCRT-I), PI3P, and components of autophagy machinery upon starvation. (A and B) Grh1-GFP was coexpressed with Vps23-mCherry (A) or with Snf7-RFP (B), cultured in growth conditions or nutrient starved for 4 h, and visualized by fluorescence microscopy. The zoom images of A represent high magnification images. (C and D) Grh1-GFP was coexpressed with RFP-Atg9, mCherry-Atg8, and a DsRed-tagged FYVE domain and cultured in growth conditions (C) or nutrient starved (D) and visualized by fluorescence microscopy. The right images represent one of the CUPS at high magnification. (E) The percentages of colocalization were quantified with respect to Grh1 or the indicated marker. At least 60 cells per marker were assessed, and errors are represented as SEM. Bars, 2 µm.
Figure 4.

Grh1 colocalizes with Vps23 (ESCRT-I), PI3P, and components of autophagy machinery upon starvation. (A and B) Grh1-GFP was coexpressed with Vps23-mCherry (A) or with Snf7-RFP (B), cultured in growth conditions or nutrient starved for 4 h, and visualized by fluorescence microscopy. The zoom images of A represent high magnification images. (C and D) Grh1-GFP was coexpressed with RFP-Atg9, mCherry-Atg8, and a DsRed-tagged FYVE domain and cultured in growth conditions (C) or nutrient starved (D) and visualized by fluorescence microscopy. The right images represent one of the CUPS at high magnification. (E) The percentages of colocalization were quantified with respect to Grh1 or the indicated marker. At least 60 cells per marker were assessed, and errors are represented as SEM. Bars, 2 µm.

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