Figure 2. Grh1 localizes near ER exit sites and relocalizes to cup-shaped structures upon starvation. (A–L) Immunoelectron microscopy of ultrathin cryosections of yeast cells. Growth conditions (A–E), 4-h starvation conditions (G–L), and conventional morphology at 4-h starvation (F) are shown. (A–C) Double immunogold labeling of Grh1-GFP (12 nm) and Sec13-RFP (6 nm). (D) Single labeling of Grh1-GFP. (E) Single labeling of Sec13-RFP. (F) Conventional morphology. (G–J) Labeling of Grh1-GFP. (K and L) Double labeling of Grh1-GFP (12 nm) and Sec13-RFP (6 nm). Arrowheads indicate 6-nm gold particles. Arrows indicate membrane lamellae. Asterisks indicate preautophagosomal structure. pm, plasma membrane; v, vacuole. Bars: (A–D, G–I, K, and L) 200 nm; (F) 500 nm; (E and J) 100 nm.
Figure 2.

Grh1 localizes near ER exit sites and relocalizes to cup-shaped structures upon starvation. (A–L) Immunoelectron microscopy of ultrathin cryosections of yeast cells. Growth conditions (A–E), 4-h starvation conditions (G–L), and conventional morphology at 4-h starvation (F) are shown. (A–C) Double immunogold labeling of Grh1-GFP (12 nm) and Sec13-RFP (6 nm). (D) Single labeling of Grh1-GFP. (E) Single labeling of Sec13-RFP. (F) Conventional morphology. (G–J) Labeling of Grh1-GFP. (K and L) Double labeling of Grh1-GFP (12 nm) and Sec13-RFP (6 nm). Arrowheads indicate 6-nm gold particles. Arrows indicate membrane lamellae. Asterisks indicate preautophagosomal structure. pm, plasma membrane; v, vacuole. Bars: (A–D, G–I, K, and L) 200 nm; (F) 500 nm; (E and J) 100 nm.

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