Figure S5.
Autophagosomes in hippocampal axons mature slowly.(A–F) Example kymographs and quantification of LC3 comigrating with Rab7 (A and B), LAMP1 (C and D), or LysoTracker (LysoTr) Deep Red dye (E and F) in different subaxonal regions. n = 8–12 neurons. Two-way ANOVA with Tukey’s multiple comparisons test (distal vs. mid: Rab7, P = 0.9956; LAMP1, P = 0.4166; LysoTracker, P = 0.0709; distal vs. proximal: Rab7, P = 0.9088; LAMP1, P = 0.0264; LysoTracker, P = 0.0280; mid vs. proximal: Rab7, P = 0.8655; LAMP1, P = 0.3205; LysoTracker, P = 0.8623.) (G and H) Example micrographs with line scans and quantification demonstrating RILP association with autophagosomes of differing maturity. n = 8–9 neurons. Two-way ANOVA with Sidak’s multiple comparisons test (mid, P = 0.4894; proximal, P = 0.0403). Dashed yellow lines indicate line scan. Scale bar, 1 µm. Bars throughout show mean ± SEM. *, P < 0.05.

Autophagosomes in hippocampal axons mature slowly. (A–F) Example kymographs and quantification of LC3 comigrating with Rab7 (A and B), LAMP1 (C and D), or LysoTracker (LysoTr) Deep Red dye (E and F) in different subaxonal regions. n = 8–12 neurons. Two-way ANOVA with Tukey’s multiple comparisons test (distal vs. mid: Rab7, P = 0.9956; LAMP1, P = 0.4166; LysoTracker, P = 0.0709; distal vs. proximal: Rab7, P = 0.9088; LAMP1, P = 0.0264; LysoTracker, P = 0.0280; mid vs. proximal: Rab7, P = 0.8655; LAMP1, P = 0.3205; LysoTracker, P = 0.8623.) (G and H) Example micrographs with line scans and quantification demonstrating RILP association with autophagosomes of differing maturity. n = 8–9 neurons. Two-way ANOVA with Sidak’s multiple comparisons test (mid, P = 0.4894; proximal, P = 0.0403). Dashed yellow lines indicate line scan. Scale bar, 1 µm. Bars throughout show mean ± SEM. *, P < 0.05.

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