Proteomic analysis of KIF4^OFF and SMC2^OFF isolated chromosomes. (A) Dependency of proteins on KIF4 for their association with mitotic chromosomes. Proteins reduced in mitotic chromosomes isolated from KIF4^OFF cells (relative to those on chromosomes isolated from wild-type cells) appear lower on the y axis. Proteins enriched on chromosomes relative to cytoplasm appear to the right on the x axis. This is Classifier II of Ohta et al. (2010a), and uses values from those experiments. Proteins most strongly depleted in this experiment include the chromatin proteins HMGN2 (a), HMGN1 (b), DEK (c), H3.3 (d), and H1.03 (e) and cytoplasmic proteins cytidine deaminase (f) and CKS1B (g). (B) Similar analysis of SMC2 dependency based on the published proteomic analysis of isolated chromosomes from wild-type and SMC2^OFF cells (Ohta et al., 2010a). (C and D) Diagrams showing the dependency of selected chromosomal (C) and centromeric (D) proteins for their association with isolated mitotic chromosomes in the presence and absence of KIF4. Bars point downward when the corresponding protein is decreased in KIF4^OFF chromosomes.