KIF4 partially colocalizes with SMC2 and topo IIα and affects condensin localization on the chromosome axis. (A) SMC2^OFF cells expressing SMC2-TrAP were stained with guinea pig anti-topo IIα, mouse anti-SBP, and rabbit anti-KIF4 plus DAPI for the DNA. Bar, 5 µm. (B and C) Asynchronously growing SMC2^OFF:SMC2-GFP-STS cells were transfected with either control or KIF4 siRNA oligos. After 20–24 h, these cells were fixed in PFA/PBS and stained by anti–CENP-T antibody and DAPI. Bars, 2 µm. (D) Quantification of B and C. Intensity of SMC2-GFP signal was measured at 5 kinetochores (Kt), 5 chromosome arms (Arm), and 5 areas in the cytosol in each of 20 cells. (E–G) Asynchronously growing SMC2^OFF:SMC2-GFP-STS cells (E), CAP-H^OFF:CAP-H-GFP-TrAP cells (F), or CAP-D3^OFF:CAP-D3-GFP-TrAP cells (G) were treated as in B and C and stained with DAPI. Bars, 2 µm.