Dynamics of internally tagged forms of KIF3A and Gag in primary macrophages. General view of a macrophage coinfected with HIV-1 Gag-iGFP ΔEnv and KIF3A-iCherry lentiviral vector and cultured for 5 d. The cell was imaged for 5 h by spinning disc microscopy. (A) Z-projection of the overlaid transmission, GFP and mCherry fluorescent intensities at time 0 (left, bar, 5 µm). The red square region is magnified for GFP and mCherry channels and the overlay of the two (panels 2–4; bar, 2 µm). Time 0, 1, 2, and 3 h 40 of a 3D reconstruction of the boxed area are presented with the tracking of the VCCs visible on the 3 h 40 panel (see Videos 3 and 4). Bar, 2 µm.