KIF3A-iCh is recruited to active VCCs. (A) Schematic representation of the KIF3A-iCh construct. (B and C) Correlative light and electron microscopy. (B) Primary macrophages grown on coverslips with coordinates were coinfected with HIV-1 Gag-iGFP and KIF3A-iCherry lentiviral vector, fixed, and imaged by spinning disk microscopy at d 7 p.i. Bar, 5 µm. (C) The same coverslips were then embedded in Epon and processed for EM. An overview of the macrophage imaged in B is presented in the top left inset. Example of mature and immature virions are presented in the bottom left inset. Bar, 100 nm. The VCCs are in the top third of the cell. (D) Two magnifications of the VCC-rich region, with a viral budding profile shown in the inset (bar, 200 nm).