KIF3A depletion results in intracellular accumulation of VCCs. (A) Examples of 3D reconstructions obtained after thresholding of image stacks acquired by confocal microscopy of HIV-1–infected macrophages transfected with the indicated siRNA and stained for Gag. (B–E) Effects of KIF3A depletion on the indicated parameters within HIV-1–infected macrophages. Reconstructions as seen in A were segmented and quantified using Imaris software. KIF3A knockdown was estimated in parallel by immunoblot in the eight donors used for these experiments. The average efficiency of silencing was 72.9%, ranging from 55 to 95%. n.s., not statistically significant.