Figure 5. Increasing chromosome number exacerbates the mitotic defects of csi1Δ cells. (A) Time-lapse microscopy of Sid4-RFP and Mis6-GFP during mitosis. Both cells have Ch16. (B) The distribution of time to finish anaphase A. All cells have Ch16. n represents the number of mitosis counted from a single experiment. (C) Serial dilution analysis of cells containing different numbers of chromosomes. Ch16H and Ch16N are modified versions of Ch16 that confer resistance to hygromycin and nourseothricin, respectively. (D) The segregation of chromosome 2 was analyzed through microscopic examination of a strain containing a LacO array inserted near the centromere of chromosome 2 that is also expressing LacI-GFP. n represents the number of cells counted from a single experiment. WT, wild type. Bar, 1 µm.
Figure 5.

Increasing chromosome number exacerbates the mitotic defects of csi1Δ cells. (A) Time-lapse microscopy of Sid4-RFP and Mis6-GFP during mitosis. Both cells have Ch16. (B) The distribution of time to finish anaphase A. All cells have Ch16. n represents the number of mitosis counted from a single experiment. (C) Serial dilution analysis of cells containing different numbers of chromosomes. Ch16H and Ch16N are modified versions of Ch16 that confer resistance to hygromycin and nourseothricin, respectively. (D) The segregation of chromosome 2 was analyzed through microscopic examination of a strain containing a LacO array inserted near the centromere of chromosome 2 that is also expressing LacI-GFP. n represents the number of cells counted from a single experiment. WT, wild type. Bar, 1 µm.

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