Csi1 is at the SPB–kinetochore interface. (A) Live-cell imaging of cells expressing Csi1-mCherry and indicated GFP fusions of kinetochore proteins. (B) A diagram of the fission yeast centromere region of chromosome 1. (C) ChIP analyses of Csi1-Flag levels at cnt and otr. (D) ChIP analyses of Csi1-Flag levels at cnt. Cells were grown at 37°C for 4 h before ChIP analysis was performed. (E) Live-cell imaging of cells expressing Csi1-GFP and Sid4-mRFP in a cnp1-1 mutant after 4 h at 37°C. (F) Live-cell imaging of cells expressing Csi1-mCherry and Mis6-GFP in a sad1.1 mutant after 3 h at 37°C. (G) Cell lysates from the indicated strains were immunoprecipitated with the Flag antibody to isolate Csi1-Flag. The associated proteins were detected by Western blot analyses with myc or HA antibodies. WCE, whole-cell extract. (H) ChIP analyses of Sad1-HA protein levels at cnt. (I) Schematic diagram of SPB–centromere organization. Error bars represent standard deviations of three experiments. DIC, differential interference contrast; IP, immunoprecipitation; WT, wild type. Bars, 1 µm.