Csi1 is required for centromere clustering during interphase. (A) Cells containing Ch16 were grown as single colonies to measure chromosome loss rate, shown below each picture. n indicates total number of colonies counted. (B) Live-cell imaging of Mis6-GFP in wild-type and csi1Δ cells. Sid4-mRFP and mRFP-Atb2 were used to confirm that cells are at interphase. (C) Quantification of the number of Mis6 foci in interphase cells. n represents the number of cells counted from a single experiment. (D) ChIP analysis of Mis6 and Cnp1 levels at centromeres (cnt). Error bars represent standard deviations of three experiments. DIC, differential interference contrast; WT wild type. Bars, 1 µm.