Figure 6. Dimerization of Dsg2.ICS-Fv1E leads to decreased internalization. (A) Schematic representation of the regulated homodimerization system. One copy of FKBP36v and HA epitope was placed in tandem with the C terminus of Dsg2-ICS. The dimerizer is AP20187. (B) SCC68 cells expressing Dsg2.ICS-Fv1E were pretreated with ethanol (control) or 10 nM AP20187 (AP) at 4°C for 30 min, and a biotinylation assay at 1.0 mM Ca2+ was then performed. Samples were immunoblotted with the anti-HA antibody. Graph presents collective results from three independent experiments. The bottom and top of the box are the 25th and 75th percentile (the lower and upper quartiles), respectively. The horizontal line near the middle of the box is the 50th percentile (the median). The ends of the whisker are the minimum and maximum of all the data. L, surface pool of the Dsg2 mutants. S, residual Dsg2 mutant protein left on the cell surface after stripping.
Figure 6.

Dimerization of Dsg2.ICS-Fv1E leads to decreased internalization. (A) Schematic representation of the regulated homodimerization system. One copy of FKBP36v and HA epitope was placed in tandem with the C terminus of Dsg2-ICS. The dimerizer is AP20187. (B) SCC68 cells expressing Dsg2.ICS-Fv1E were pretreated with ethanol (control) or 10 nM AP20187 (AP) at 4°C for 30 min, and a biotinylation assay at 1.0 mM Ca2+ was then performed. Samples were immunoblotted with the anti-HA antibody. Graph presents collective results from three independent experiments. The bottom and top of the box are the 25th and 75th percentile (the lower and upper quartiles), respectively. The horizontal line near the middle of the box is the 50th percentile (the median). The ends of the whisker are the minimum and maximum of all the data. L, surface pool of the Dsg2 mutants. S, residual Dsg2 mutant protein left on the cell surface after stripping.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal