Figure 4. Internalization of Dsg2 mutants depends on cholesterol and dynamin. (A) Biotinylation assay of cells that were pretreated with 5 mM methyl-β-cyclodextrin (m-β-C) for 30 min. L, surface pool of the Dsg2 mutants. S, residual Dsg2 mutant protein left on the cell surface after stripping. (B) Cells grown in medium with 0.25 mM Ca2+ were treated with 80 µM dynasore for 30 min and then stained for FLAG. Bar, 20 µm.
Figure 4.

Internalization of Dsg2 mutants depends on cholesterol and dynamin. (A) Biotinylation assay of cells that were pretreated with 5 mM methyl-β-cyclodextrin (m-β-C) for 30 min. L, surface pool of the Dsg2 mutants. S, residual Dsg2 mutant protein left on the cell surface after stripping. (B) Cells grown in medium with 0.25 mM Ca2+ were treated with 80 µM dynasore for 30 min and then stained for FLAG. Bar, 20 µm.

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