USH2 proteins in mouse and macaque photoreceptors. (A) Predicted structures of USH2 proteins. The positions of the recombinant proteins used to engineer the new antibodies (in bold) are indicated. (B) In both the mouse (top panels) and macaque (bottom panels) photoreceptors, usherin, Vlgr1, and whirlin are detected (green) in the periciliary membrane complex (PMC) region, around the connecting cilium (CC, highlighted in red in the schematic diagram). The inset in B (middle) shows immunogold labeling of Vlgr1 in the PMC region. Cell nuclei are stained with DAPI (blue). IS, inner segment; OS, outer segment. Bars: 5 µm; (B, inset) 250 nm.