USH1 transcripts in the murine retina. (A) RT-PCR analysis of USH1 gene expression in the neural retina (NR) and the retinal pigment epithelium cells (RPE) of mice, humans, and macaques. In each case, a negative control without reverse transcription (−RT) was performed. (B) Transcriptomic analysis of USH1 and USH2 gene expression levels in the neural retina of wild-type mice. Note that sans transcripts are absent from the Affymetrix gene chips, and the relative levels of Cdh23 and Ush1c transcripts are much lower than those of other USH1 and USH2 gene transcripts. (C) Quantitative RT-PCR analysis of the relative levels of USH1 and USH2 gene transcript expression in the neural retinas of wild-type and rd1 P15 mice. The significantly lower abundance of the Myo7a and Pcdh15, Ush2a, Gpr98 and Whrn transcripts in the retinas of rd1 P15 mice indicates that the corresponding genes are expressed in mouse rod photoreceptors. **, P < 0.01; ***, P < 0.001 in Student’s t test.