Figure 4.
![Figure 4. Folding of mtHsp70 in vivo requires Hep1. Δhep1 and wild-type (wt) cells were radiolabeled with [35S]methionine for 10 min. Then protein synthesis was stopped and mitochondria were isolated and solubilized. The lysate was incubated without and with trypsin to assess the folding of mtHsp70. mtHsp70 was immunoprecipitated with antibodies against mtHsp70. Samples were analyzed by SDS-PAGE and autoradiography (left) and quantified by densitometry (right). Total material (−Trypsin) was set to 100%, and trypsin-resistant material is shown as the percentage of total material. The diagram represents the mean values ± standard deviation (n = 3).](https://cdn.rupress.org/rup/content_public/journal/jcb/199/1/10.1083_jcb.201205012/4/jcb_201205012_fig4.jpeg?Expires=1773568293&Signature=dtqPw8JEFIC2OHG6X~D7g3jNe6BA~OpVkbgs-qJ2LO5961yQlsKxLqg~ZHkUq2~o1wBY0L80~Bvsr9w6ptozk5ydZ-nbuPhKbF3KE1o8Avy-ZlTXWTxkSmd-sUFan7ozIFrVjIsQb1GqjuWN3zBVuwmE8TH2me2jh3eNMMBxxVGnxkz916kuCIIJrenwpdGi2ACUBFXRW55hBCPwXGyrxiirfk55KWlRQM3u3Z-0L7YdSlAWhW61JkGn1gudZqejDyK~oPfyMdWJ-Lf75HualNxZEYpjpCPorB-Ylib2b1fFCEnctG-KmiaEJvC2LjgopR5rhhCnQ03HKM9VM8pdhA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Folding of mtHsp70 in vivo requires Hep1. Δhep1 and wild-type (wt) cells were radiolabeled with [35S]methionine for 10 min. Then protein synthesis was stopped and mitochondria were isolated and solubilized. The lysate was incubated without and with trypsin to assess the folding of mtHsp70. mtHsp70 was immunoprecipitated with antibodies against mtHsp70. Samples were analyzed by SDS-PAGE and autoradiography (left) and quantified by densitometry (right). Total material (−Trypsin) was set to 100%, and trypsin-resistant material is shown as the percentage of total material. The diagram represents the mean values ± standard deviation (n = 3).
