Effects of lowering myosin contractility in cells with reduced cell–cell adhesions. (A and B) Normalized cell pair separation distance (A) and velocity correlation (B) for control cells, cells treated with siCTNNA1, MIIA knocked down cells, and MIIA knocked down cells treated with siCTNNA1 on stiff (65 kPa) substrates. (C) Velocity correlation for control and siCDH3-treated cells on glass after blebbistatin treatment. Inset shows velocity correlation at R = 225 µm for siCDH3 cells. N = number of experiments. All nonoverlapping error bars (95% SEM) are statistically significant with P < 0.05. For the inset in C, statistical significance was specifically calculated for 0, 5, and 10 µM blebbistatin treatments to highlight the partial rescue in velocity correlation with low dose blebbistatin treatment. **, P < 0.005; ***, P < 0.0001.