The effects of siCDH3 and siCTNNA1 on actin organization and pMLC level in cells during wound healing. (A) Maximum projections of confocal immunofluorescence images of pMLC (green) costained with phalloidin (red) and DAPI (blue) in control and knockdown cells at the wound edge and within the migrating cell monolayer. Insets are numbered and magnified at the right with corresponding maximum projection E-cadherin immunofluorescence images. Bars, 50 µm. (B) Quantification of pMLC fluorescence intensity per cell, normalized to that of the control wound edge cells on stiff substrates. (C) pMLC fluorescence intensity per cell on stiff (65 kPa) substrates, normalized to that of wound edge cells for each condition. Error bars show SEM. N = number of independent experiments. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.