The effects of P-cadherin knockdown (siCDH3) on migration properties during wound healing. (A) Effects of siCDH3 treatment on mean distance advanced by the cell sheet. (B–F) Migration properties of siCDH3-treated cells undergoing wound healing on soft (3 kPa) and stiff (65 kPa) substrates, compared with control cells, plotted at same time points after wounding and distances from wound edge as in Figs. 1 and 5. (G and H) Golgi orientation at 12 h after wounding on soft (3 kPa) or stiff (65 kPa) substrates. N = number of experiments; n = total number of cells measured from N experiments. All nonoverlapping error bars (95% SEM) are statistically significant with P < 0.05.