Myosin IIA accumulates at cell junctions upon microtubule stabilization. (A and B) Myosin IIA (MHCIIA, green in all images) localizes weakly at junctions and throughout the cytoplasm in control cells (A). In taxol-treated cells, myosin IIA junctional localization increases (B). (C and D) Myosin IIA accumulates at cell junctions of cells transfected with Tau-GFP (starred cells in D), but not in GFP-transfected cells (starred in C). (E and F) Myosin IIA does not accumulate at cell junctions after taxol treatment in desmoplakin-null cells. (G–J) Myosin IIA accumulation at cell junctions in response to taxol treatment is not affected by pharmacologic inhibition of myosin II or Rho kinase (blebbistatin or Y27632 treatment in H and J). Bars,10 µm.