Figure 5. Spindles adopt a fixed but incorrect orientation in CHICA-, HMMR-, or DYNLL1-depleted cells. HeLa cells stably expressing GFP-tubulin and mCherry-histone H2B were transfected with the siRNA duplexes shown in the figure for 72 h, and the full cell volume was imaged on an Ultraview spinning-disk confocal microscope every minute as the cells passed through mitosis. The angle of spindle rotation was measured and is plotted in the graph as a function of time for three individual cells. The point at which bipolar spindle formation was complete is shown by the dotted line. A red circle marks the time point when chromosome segregation was first observed, taken as a marker for the onset of anaphase. Three example curves are shown taken from a single representative experiment of five replicates.
Figure 5.

Spindles adopt a fixed but incorrect orientation in CHICA-, HMMR-, or DYNLL1-depleted cells. HeLa cells stably expressing GFP-tubulin and mCherry-histone H2B were transfected with the siRNA duplexes shown in the figure for 72 h, and the full cell volume was imaged on an Ultraview spinning-disk confocal microscope every minute as the cells passed through mitosis. The angle of spindle rotation was measured and is plotted in the graph as a function of time for three individual cells. The point at which bipolar spindle formation was complete is shown by the dotted line. A red circle marks the time point when chromosome segregation was first observed, taken as a marker for the onset of anaphase. Three example curves are shown taken from a single representative experiment of five replicates.

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