Figure 1. DYNLL1-dynein associates with a defined subset of spindle proteins in mitosis. (A) Control and DYNLL1 complexes were analyzed by SDS-PAGE and the major Coomassie blue–stained proteins identified by mass spectrometry are marked, or (B) by Western blotting using the specific antibodies shown in the figure. CHICA-C antibody was used for blotting. Asterisks mark the antibody heavy and light chains. (C) Complexes were immunoprecipitated using HMMR, CHICA-N, and HURP antibodies from HeLa cells arrested in mitosis using 200 ng/ml nocodazole for 18 h before cell lysis. Antibodies to GFP were used as a negative control. The immunoprecipitates were Western blotted using the antibodies shown in the figure. CHICA-C antibody was used for blotting. The asterisk marks light chain cross reactivity in the kinastrin blot.
Figure 1.

DYNLL1-dynein associates with a defined subset of spindle proteins in mitosis. (A) Control and DYNLL1 complexes were analyzed by SDS-PAGE and the major Coomassie blue–stained proteins identified by mass spectrometry are marked, or (B) by Western blotting using the specific antibodies shown in the figure. CHICA-C antibody was used for blotting. Asterisks mark the antibody heavy and light chains. (C) Complexes were immunoprecipitated using HMMR, CHICA-N, and HURP antibodies from HeLa cells arrested in mitosis using 200 ng/ml nocodazole for 18 h before cell lysis. Antibodies to GFP were used as a negative control. The immunoprecipitates were Western blotted using the antibodies shown in the figure. CHICA-C antibody was used for blotting. The asterisk marks light chain cross reactivity in the kinastrin blot.

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