Silencing of miR-24 targets, PAK4, Tks5, and ArhGAP19 phenocopies miR-24 overexpression in cells. (A) Western blot analysis confirmed silencing of PAK4, Tsk5, and ArhGAP19. Tubulin was used as a loading control. (B) Silencing of PAK4, Tsk5, and ArhGAP19 causes remodeling of actin filaments. (C) Specific silencing of PAK4, Tsk5, or ArhGAP19 induces loss of planar polarization in HEKn as shown by Alexa Fluor 488–phallotoxin (green) and paxillin staining. (D) HEKn were fixed and stained with Alexa Fluor 488–phallotoxin (green) and E-cadherin (E-Cadh) antibody to highlight the formation of AJs. MM, molecular mass; Scr, scramble. Bars: (B) 20 µm; (C) 10 µm; (D) 30 µm.