RPGRIP1L stabilizes dishevelled at the cilium base in cultured mammalian renal cells. (A) Co-immunostaining of Dvl2 (top) or Dvl3 (middle) with γ-tubulin on control and RPGRIP1L-KD MDCK cells. The bottom panel shows z-section of coimmunostaining of Dvl3 and γ-tubulin in the three cell lines. The histogram compares the percentage of cells with Dvl2 or Dvl3 staining and their accumulation in the pericentriolar region versus the cytoplasm (800–900 cells analyzed). (B) Quantitative RT-PCR analysis of Dvl2 and Dvl3 mRNA levels in control and RPGRIP1L-KD MDCK cells. (C) Western blot analysis and quantification of endogenous Dvl2 and Dvl3 in control and RPGRIP1L-KD MDCK cells. (D) Coimmunostaining of inversin with γ-tubulin on control and RPGRIP1L-KD MDCK cells. The right panel shows z-section of the stainings in both cell lines. (E) Immunostaining of NPHP4-V5 on control and RPGRIP1L-KD MDCK cells transduced with the NPHP4-V5 expression construct (Burcklé et al., 2011). In A, D, and E nuclei were stained with Hoechst. Bars: (C, D, z-sections A or C) 10 µm; (A, top and middle panels) 2 µm.