The pull-down of RSP3_1–178 contains LC8, RSPs, and putative RS-docking proteins. (A and B) Western blots (A) and silver-stained protein gels of the Ni-NTA pull-down (B) from the KI axonemal extracts of pf14 and the pf14 transformant that expresses RSP3_1–178-HAHis. The RSPs in the RSP3_1–178 pull-down were LC8 and the RIIa domain–containing RSP7 (B, right) and RSP11 (A and B, right blots). An unknown 85-kD doublet (arrow) and CaM-IP2, -IP3, and -IP4 (dots) in the CSC were also in the pull-down but were scarce or undetectable in the pull-down from pf14. CaM and IC140 in the inner dynein arm I1 were not pulled down appreciably. CaM-IP4 was identified based on its copurification with CaM-IP2 and CaM-IP3 and its molecular mass. Pre, axonemal extract; Post, flow-through; E, eluate; Tub, tubulin.