Figure 2.
Figure 2. C-cadherin surface expression is not affected by Wnt-11 and Fz7. Time-lapse microscopic analysis of xC-cadherin subcellular localization during convergent extension. 16-cell–stage embryos were injected into the DMZ region with 500 pg xC-cadherin–EGPF RNA alone or in combination with 20 pg xWnt-11 RNA, 1 pmol xWnt-11 MO, 500 pg xFz7 RNA, or 1.6 pmol xFz7 MO. 200 pg GAP43-mCherry RNA was coinjected in all samples as a cell membrane tracer. At stage 10.25, DMZ explants were cut, and subcellular localization of xC-cadherin–EGFP was analyzed. (A–E) Representative images of confocal time-lapse videos are shown. Neither overexpression nor depletion of xWnt-11 or xFz7 had an effect on xC-cadherin–EGFP subcellular localization. Bars, 20 µm.

C-cadherin surface expression is not affected by Wnt-11 and Fz7. Time-lapse microscopic analysis of xC-cadherin subcellular localization during convergent extension. 16-cell–stage embryos were injected into the DMZ region with 500 pg xC-cadherin–EGPF RNA alone or in combination with 20 pg xWnt-11 RNA, 1 pmol xWnt-11 MO, 500 pg xFz7 RNA, or 1.6 pmol xFz7 MO. 200 pg GAP43-mCherry RNA was coinjected in all samples as a cell membrane tracer. At stage 10.25, DMZ explants were cut, and subcellular localization of xC-cadherin–EGFP was analyzed. (A–E) Representative images of confocal time-lapse videos are shown. Neither overexpression nor depletion of xWnt-11 or xFz7 had an effect on xC-cadherin–EGFP subcellular localization. Bars, 20 µm.

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