Figure 6. PHD12 protein interacts in... | Rockefeller University Press

Figure 6.

$Figure 6. PHD12 protein interacts in vivo with Sin3A and indirectly with Snail2 as revealed by co-IP and BiFC. (A) Western blots using anti-Snail2 and -Sin3A on PHD12 or IgG immunoprecipitates (IP) from dorsal neural tubes from 6–8ss embryos. Input represent 0.1% of each IP. (B) Schematic representation of PHD12 constructs used for the bimolecular fluorescence complementation (BiFC) assay. (C and D) Visualization and quantification of BiFC on chick fibroblast transfected with PHD12 and truncated PHD12, Snail2, and Sin3A fused to the C or N terminus of Venus protein. Y axis on graph represents the fraction of transfected cells in which Venus fluorescence was visualized. Ct, C terminus; Nt, N terminus; FHA, Forkhead-associated domain; VC, Venus C terminus; VN, Venus N terminus; WB, Western blot. Error bars show SDs. Bar, 16 µm.$

PHD12 protein interacts in vivo with Sin3A and indirectly with Snail2 as revealed by co-IP and BiFC. (A) Western blots using anti-Snail2 and -Sin3A on PHD12 or IgG immunoprecipitates (IP) from dorsal neural tubes from 6–8ss embryos. Input represent 0.1% of each IP. (B) Schematic representation of PHD12 constructs used for the bimolecular fluorescence complementation (BiFC) assay. (C and D) Visualization and quantification of BiFC on chick fibroblast transfected with PHD12 and truncated PHD12, Snail2, and Sin3A fused to the C or N terminus of Venus protein. Y axis on graph represents the fraction of transfected cells in which Venus fluorescence was visualized. Ct, C terminus; Nt, N terminus; FHA, Forkhead-associated domain; VC, Venus C terminus; VN, Venus N terminus; WB, Western blot. Error bars show SDs. Bar, 16 µm.