Figure 4. Docking of the Kar3Vik1 crystal structure to the cryo-EM data. Docking of the cross-linked SHD-Kar3Vik1 crystal structure into the three-dimensional maps obtained from helical reconstruction of WT GCN4-Kar3Vik1–decorated microtubules in the nucleotide-free (A and B) and AMPPNP (C and D) states. A and C show views along the length of the protofilament, with the minus end of the microtubule pointing to the right. B and D provide views through the cross section of the microtubule with the minus end facing away from the reader. Kar3, Vik1, the SHD domain, α-tubulin, and β-tubulin are depicted in gray, yellow, pale blue, light turquoise, and dark turquoise, respectively. Helix α4 of Kar3 is depicted in red in all the maps to orient the reader. In C and D, the docked position of Kar3Vik1 in the nucleotide-free state is shown in transparent gray.
Figure 4.

Docking of the Kar3Vik1 crystal structure to the cryo-EM data. Docking of the cross-linked SHD-Kar3Vik1 crystal structure into the three-dimensional maps obtained from helical reconstruction of WT GCN4-Kar3Vik1–decorated microtubules in the nucleotide-free (A and B) and AMPPNP (C and D) states. A and C show views along the length of the protofilament, with the minus end of the microtubule pointing to the right. B and D provide views through the cross section of the microtubule with the minus end facing away from the reader. Kar3, Vik1, the SHD domain, α-tubulin, and β-tubulin are depicted in gray, yellow, pale blue, light turquoise, and dark turquoise, respectively. Helix α4 of Kar3 is depicted in red in all the maps to orient the reader. In C and D, the docked position of Kar3Vik1 in the nucleotide-free state is shown in transparent gray.

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