Figure 3. Structure of cross-linked SHD-Kar3Vik1 and comparison to Ncd. (A) A ribbon representation of the structure of cross-linked SHD-Kar3Vik1 (PDB accession no. 4ETP) solved to a resolution of 2.3 Å by x-ray crystallography, where the SHD is shown in pale cyan, Vik1 in yellow, and Kar3 in white. Kar3 is complexed to MgADP, which is shown in the space filling representation. Kar3′s helix α4 is denoted as a point of reference. (B) Vik1 contains a 12-Å intramolecular cross-link (EBI) between its globular domain (K423C) and helix (E355C). Electron density for the cross-link is shown at a contour level of 1σ. (C) The structure of cross-linked SHD-Kar3Vik1 shows striking similarity to the asymmetric structures of Ncd (PDB accession nos. 1N6M and 3L1C; Yun et al., 2003; Heuston et al., 2010). The active sites of Ncd contain MgADP, shown in space filling representation. Fig. 3 was prepared with Pymol.
Figure 3.

Structure of cross-linked SHD-Kar3Vik1 and comparison to Ncd. (A) A ribbon representation of the structure of cross-linked SHD-Kar3Vik1 (PDB accession no. 4ETP) solved to a resolution of 2.3 Å by x-ray crystallography, where the SHD is shown in pale cyan, Vik1 in yellow, and Kar3 in white. Kar3 is complexed to MgADP, which is shown in the space filling representation. Kar3′s helix α4 is denoted as a point of reference. (B) Vik1 contains a 12-Å intramolecular cross-link (EBI) between its globular domain (K423C) and helix (E355C). Electron density for the cross-link is shown at a contour level of 1σ. (C) The structure of cross-linked SHD-Kar3Vik1 shows striking similarity to the asymmetric structures of Ncd (PDB accession nos. 1N6M and 3L1C; Yun et al., 2003; Heuston et al., 2010). The active sites of Ncd contain MgADP, shown in space filling representation. Fig. 3 was prepared with Pymol.

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