Normal loading of DMC1 and RPA accumulation at leptonema-arrested dKO-kls spermatocytes. Double immunolabeling of SYCP3 and DMC1 or RPA. (A) In wild-type and dKO-kls spermatocytes, DMC1 protein localizes to AEs/LEs at leptonema. (B) In dKO-kls spermatocytes, there is a threefold increase in the numbers of RPA foci at the leptotene-like arrest in comparison with wild-type spermatocytes. Bars, 100 µm.