Mxc and FLASH remain chromosome associated during mitosis. (A–D) Postblastoderm w¹¹¹⁸ embryos in cell cycle 14 stained with α–P-Tyr, α-PH3 (marks condensed, mitotic chromatin), DAPI, and α-Mute-LS (A), α-GFP (B), α-Mxc (C), or α-FLASH (D). Prophase (pro; white arrows), metaphase (meta; white arrowheads), anaphase (ana; yellow arrowheads), and telophase (telo; yellow arrows) cells are indicated. Bars, 10 µm. (A′–D′) Single prophase, metaphase, anaphase, and telophase cells are shown in A′, B′, C′, and D′. Bars, 5 µm. Note that the mouse α-PH3 used in D stains interphase cells weakly.